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Position: Home > issue > May 10, 2022 Volume 3, Issue 3
Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing
Category:   Article   Download:  PDF  Figure  Endnote
Author: Yangcan Chen, Yanping Hu, Xinge Wang, Shengqiu Luo, Ning Yang, Yi Chen, Zhikun Li, Qi Zhou, Wei Li

c3.jpg
GRAPHICAL ABSTRACT


The naturally occurring prokaryotic CRISPR-Cas systems provide valuable resources for the development of new genome-editing tools. However, the majority of prokaryotic Cas nucleases exhibit poor editing efficiency in mammalian cells, which significantly limits their utility. Here, we have developed a method termed Improving Editing Activity by Synergistic Engineering (MIDAS). This method exerts a synergistic effect to improve mammalian genome-editing efficiency of a wide range of CRISPR-Cas systems by enhancing the interactions between Cas nuclease with the protospacer adjacent motif (PAM) and the single-stranded DNA (ssDNA) substrate in the catalytic pocket simultaneously.





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