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On the cover: The pace of science and technology is accelerating, and the future is arriving much faster. During the great process of reaching the future, the now highly comprehensive and interdisciplinary network of science, so-called 'ScienceX' by The Innovation, plays a more important role than ever before. We believe the development of interdisciplinary science and technology concerns our destiny in the coming future. |
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Position: Home > issue > May 10, 2022 Volume 3, Issue 4 |
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Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing |
Category: Article Download: PDF Figure Endnote |
Author: Yangcan Chen, Yanping Hu, Xinge Wang, Shengqiu Luo, Ning Yang, Yi Chen, Zhikun Li, Qi Zhou, Wei Li |
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 GRAPHICAL ABSTRACT
The naturally occurring prokaryotic CRISPR-Cas systems provide valuable resources for the development of new genome-editing tools. However, the majority of prokaryotic Cas nucleases exhibit poor editing efficiency in mammalian cells, which significantly limits their utility. Here, we have developed a method termed Improving Editing Activity by Synergistic Engineering (MIDAS). This method exerts a synergistic effect to improve mammalian genome-editing efficiency of a wide range of CRISPR-Cas systems by enhancing the interactions between Cas nuclease with the protospacer adjacent motif (PAM) and the single-stranded DNA (ssDNA) substrate in the catalytic pocket simultaneously.

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