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| On the cover: The sun not only breeds all life, but also brings inexhaustible clean energy to the earth. The development of flexible photovoltaic technology, represented by flexible perovskite solar cells, frees the energy production from the constraints of traditional centralized photovoltaic application scenarios. From spacecraft and curved photovoltaics to wearable devices and IoT sensors, the combination of flexible photovoltaics and advanced technology will realize a more flexible, convenient and imaginative collection and utilization of solar energy, contributing to a more intelligent and sustainable human society. |
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| Position: Home > issue > November 08, 2022 Volume 3, Issue 6 |
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| Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing |
Category: Article Download:  PDF  Figure  Endnote |
| Author: Rui Bi, Yu Li, Min Xu, Quanzhen Zheng, Deng-Feng Zhang, Xiao Li, Guolan Ma, Bolin Xiang, Xiaojia Zhu, Hui Zhao, Xingxu Huang, Ping Zheng, Yong-Gang Yao |
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Graphical abstract Pathogenic mitochondrial DNA (mtDNA) mutations can cause a variety of human diseases. The recent development of genome-editing technologies to manipulate mtDNA, such as mitochondria-targeted DNA nucleases and base editors, offer a promising way for curing mitochondrial diseases caused by mtDNA mutations. The CRISPR-Cas9 system is a widely used tool for genome editing; however, its application in mtDNA editing is still under debate. In this study, we developed a mito-Cas9 system by adding the mitochondria-targeted sequences and 3' untranslated region of nuclear-encoded mitochondrial genes upstream and downstream of the Cas9 gene, respectively.
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