A whole-cell hydrogen peroxide biosensor and its application in visual food analysis

Yi-Zhou Gao; Yun Wang; Mengmeng Ji; Ning-Yi Zhou; Wei E. Huang

doi:10.59717/j.xinn-life.2023.100011

The Innovation Life > 2023 Vol. 1 > No. 1 > 100011

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A whole-cell hydrogen peroxide biosensor and its application in visual food analysis

1.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
2.
Oxford Suzhou Centre for Advanced Research, University of Oxford, Suzhou 215000, China
3.
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
4.
The Center for Microbes, Development and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China

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Corresponding authors: ningyi.zhou@sjtu.edu.cn (N.Z.); wei.huang@eng.ox.ac.uk (W.H.)
Received Date: 23 March 2023

Accepted Date: 29 May 2023

Published Online: 29 May 2023

The Innovation Life 1(1), https://doi.org/10.59717/j.xinn-life.2023.100011 | Cite this article

GRAPHICAL ABSTRACT

GRAPHICAL ABSTRACT

PUBLIC SUMMARY

PUBLIC SUMMARY
1. A whole-cell bacterial H₂O₂ biosensor was constructed.
  
  The luminescence signal of the biosensor responsed well to different concentration of H₂O₂.
  
  With this biosensor, the H₂O₂ in solid food or milk can be detected.

ABSTRACT

ABSTRACT

Hydrogen peroxide (H₂O₂) is broadly used in the food industry for bleaching, sterilization, and deodorization. Detection of H₂O₂ in food/drinks is important for food safety. In this study, the H₂O₂-inducible whole-cell biosensor KT2440 [pPahpC] was constructed based on the bacterial strain Pseudomonas putida KT2440. The H₂O₂-inducible promoter PahpC was fused with the reporter gene cluster luxCDABE to produce an H₂O₂-inducible bioluminescent biosensor. KT2440 [pPahpC] semi-quantitatively detected H₂O₂ in the range of 10 - 2000 µM. This H₂O₂ biosensor exhibited high specificity and no response to other commonly used redox agents, such as KMnO₄, Ca(ClO)₂, and thiourea. This KT2440 [pPahpC] biosensor was used to detect H₂O₂ in food samples, demonstrating its robust performance. The whole-cell biosensor provides a new approach to the detection of H₂O₂ in the food industry.

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REFERENCES

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Gao Y.-Z., Wang Y., Ji M., et al., (2023). A whole-cell hydrogen peroxide biosensor and its application in visual food analysis. The Innovation Life 1(1), 100011. https://doi.org/10.59717/j.xinn-life.2023.100011

Gao Y.-Z., Wang Y., Ji M., et al., (2023). A whole-cell hydrogen peroxide biosensor and its application in visual food analysis. The Innovation Life 1(1), 100011. https://doi.org/10.59717/j.xinn-life.2023.100011

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A whole-cell hydrogen peroxide biosensor and its application in visual food analysis

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