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The Innovation Life > 2025 Vol. 3 > No. 2 > 100129
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Innovation of self-labeling protein tags: Engineering HaloTag for recording living cell physiology

  • 1.

    Department of Systems Science, Faculty of Arts and Sciences, Beijing Normal University, Zhuhai 519087, China

  • 2.

    International Academic Center of Complex Systems, Beijing Normal University, Zhuhai 519087, China

  • 3.

    School of Systems Science, Beijing Normal University, Beijing 100875, China

  • 4.

    Department of Biology, Faculty of Arts and Sciences, Beijing Normal University, Zhuhai 519087, China

  • 5.

    These authors contributed equally

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  • Corresponding author: kezhang@bnu.edu.cn
  • Received Date:  December 22, 2024
    Accepted Date:  March 07, 2025
    Published Online:  March 11, 2025
The Innovation Life  3(2),  https://doi.org/10.59717/j.xinn-life.2024.100129  |  Cite this article
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    • REFERENCES
  • [1] Das A., Holden S., Borovicka J., et al. (2023). Large-scale recording of neuronal activity in freely-moving mice at cellular resolution. Nat. Commun. 14:6399. DOI:10.1038/s41467-023-42083-y

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    [2] Meinen R.M., Weber J.N., Albrecht A., et al. (2021). Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta. Plant. Commun. 5:100212. DOI:10.1016/j.xplc.2021.100212

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    [3] Huppertz M.C., Wilhelm J., Grenier V., et al. (2024). Recording physiological history of cells with chemical labeling. Science 383:890−897. DOI:10.1126/science.adg0812

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    [4] Deo C., Abdelfattah A.S., Bhargava H.K., et al. (2021). The HaloTag as a general scaffold for far-red tunable chemigenetic indicators. Nat. Chem. Biol. 17:718−723. DOI:10.1038/s41589-021-00775-w

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    [5] Frei M.S., Tarnawski M., Roberti M.J., et al. (2022). Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat. Methods 19:65−70. DOI:10.1038/s41592-021-01341-x

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  • Cite this article:

    Jia H., Yang Y., Huang B., et al. (2025). Innovation of self-labeling protein tags: Engineering HaloTag for recording living cell physiology. The Innovation Life 3:100129. https://doi.org/10.59717/j.xinn-life.2024.100129
    Jia H., Yang Y., Huang B., et al. (2025). Innovation of self-labeling protein tags: Engineering HaloTag for recording living cell physiology. The Innovation Life 3:100129. https://doi.org/10.59717/j.xinn-life.2024.100129

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