Absolute quantification of the microbiota spatial distribution in the murine large intestine

Wen-Yu Jin; Jia-Xin Guo; Meng Zhang; Lin-Zuo Teng; Yanjie Chao; Philippe Joseph Sansonetti; Yi-Zhou Gao

doi:10.59717/j.xinn-life.2023.100030

The Innovation Life > 2023 Vol. 1 > No. 2 > 100030

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Absolute quantification of the microbiota spatial distribution in the murine large intestine

1.
The Center for Microbes, Development and Health, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai 200031, China

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Corresponding authors: philippe.sansonetti@siii.cas.cn (P.S.); yzgao@siii.cas.cn (Y.-Z.G.)
Received Date: July 06, 2023

Accepted Date: September 13, 2023

Published Online: September 14, 2023

The Innovation Life 1(2), https://doi.org/10.59717/j.xinn-life.2023.100030 | Cite this article

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PUBLIC SUMMARY
1. Reliable absolute quantification of microbiome based on triple spike-in bacteria.
  
  Absolute quantification of total bacteria in cecum, distal colon, and proximal colon of mice.
  
  Absolute quantification of bacteria in content and mucus layer in different part of the intestine.

ABSTRACT

ABSTRACT

The species diversity, abundance, and location of intestinal bacteria bear major consequences on host intestinal functions, affecting the balance between health and disease. Recent studies addressed the relative abundance of bacteria in feces and on the gut mucosal surface. However, few of these publications reported the absolute number of bacteria in different compartments of the gut, especially those associated with the intestinal epithelial surface, compared to the luminal content reflected by feces. In this study, the number of bacteria present in the lumen or associated with the mucosal surface of mice gut samples was calculated using the bacterial “internal standard method” during sequencing. Three spiking bacteria were used for mutual corroboration. The microbiota spatial distribution in the cecum, proximal and distal colon were studied in the lumen content and gut wall fixed niches. This study also provides an example to demonstrate that absolute and relative quantification may yield different conclusions. This study’s method may eliminate such misjudgments and provide insight into the distribution and quantity of gut microbiome.

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Jin W.-Y., Guo J.-X., Zhang M., et al., (2023). Absolute quantification of the microbiota spatial distribution in the murine large intestine. The Innovation Life 1(2), 100030. https://doi.org/10.59717/j.xinn-life.2023.100030

Jin W.-Y., Guo J.-X., Zhang M., et al., (2023). Absolute quantification of the microbiota spatial distribution in the murine large intestine. The Innovation Life 1(2), 100030. https://doi.org/10.59717/j.xinn-life.2023.100030

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Absolute quantification of the microbiota spatial distribution in the murine large intestine

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Absolute quantification of the microbiota spatial distribution in the murine large intestine

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