| [1] | Barrangou, R., Fremaux, C., Deveau, H., et al. (2007). CRISPR provides acquired resistance against viruses in prokaryotes. Science 315: 1709−1712. DOI: 10.1126/science.1138140. |
| [2] | Wang, J.Y., and Doudna, J.A. (2023). CRISPR technology: A decade of genome editing is only the beginning. Science 379: eadd8643. DOI: 10.1126/science.add8643. |
| [3] | Karvelis, T., Druteika, G., Bigelyte, G., et al. (2021). Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature 599: 692−696. DOI: 10.1038/s41586-021-04058-1. |
| [4] | Altae-Tran, H., Kannan, S., Demircioglu, F.E., et al. (2021). The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Science 374: 57−65. DOI: 10.1126/science.abj6856. |
| [5] | Zhang, H., Kong, X., Xue, M., et al. (2023). An engineered xCas12i with high activity, high specificity, and broad PAM range. Protein Cell 14: 538−543. DOI: 10.1093/procel/pwac052. |
| [6] | Duan, Z., Liang, Y., Sun, J., et al. (2024). An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plants. The Innovation 5: 100564. DOI: 10.1016/j.xinn.2024.100564. |
| [7] | Fonfara, I., Richter, H., Bratovič, M., et al. (2016). The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature 532: 517−521. DOI: 10.1038/nature17945. |
| [8] | Lv, P., Su, F., Chen, F., et al. (2024). Genome editing in rice using CRISPR/Cas12i3. Plant Biotechnol. J. 22: 379−385. DOI: 10.1111/pbi.14192. |
| [9] | Bae, S., Park, J., and Kim, J.S. (2014). Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30: 1473−1475. DOI: 10.1093/bioinformatics/btu048. |
| [10] | Liu, Q., Wang, C., Jiao, X.Z., et al. (2019). Hi-TOM: A platform for high-throughput tracking of mutations induced by CRISPR/Cas systems. Sci. China Life Sci. 62: 1−7. DOI: 10.1007/s11427-018-9402-9. |
| Han X., Chen Y., Liu R., et al., (2024). Engineering hfCas12Max for improved gene editing efficiency. The Innovation Life 2(2): 100067. https://doi.org/10.59717/j.xinn-life.2024.100067 |
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hfCas12Max**, an engineered variant hfCas12Max, mediates high-efficiency editing in mammalian cells and plants