Racial disparity in PM2.5 species and DNA methylation in CARDIA: An epigenome-wide association study

Jie Yao; Yinan Zheng; Jincheng Shen; Brian T Joyce; Ke Hao; Ravi V Shah; Venkatesh L Murthy; Stephen Sidney; Donald Lloyd-Jones; Lifang Hou; Kai Zhang

doi:10.59717/j.xinn-med.2024.100061

The Innovation Medicine > 2024 Vol. 2 > No. 2 > 100061

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Racial disparity in PM_2.5 species and DNA methylation in CARDIA: An epigenome-wide association study

1.
Department of Epidemiology and Biostatistics, School of Public Health, University at Albany, State University of New York, Rensselaer 12144, USA
2.
Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago 60611, USA
3.
Department of Population Health Sciences, University of Utah, Salt Lake City 84108, USA
4.
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York 10029, USA
5.
Vanderbilt Translational and Clinical Cardiovascular Research Center, Vanderbilt University Medical Center, Nashville 37232, USA
6.
Department of Medicine and Radiology, University of Michigan, Ann Arbor 48109, USA
7.
Division of Research, Kaiser Permanente Northern California, Oakland 94612, USA
8.
Department of Environmental Health Sciences, School of Public Health, University of Albany, State University of New York, Rensselaer 12144, USA

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Corresponding authors: l-hou@northwestern.edu (L.H.); kzhang9@albany.edu (K.Z.)
Received Date: October 31, 2023

Accepted Date: February 28, 2024

Published Online: April 01, 2024

The Innovation Medicine 2(2), https://doi.org/10.59717/j.xinn-med.2024.100061 | Cite this article

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PUBLIC SUMMARY

PUBLIC SUMMARY
1. There are statistically significant associations between DNA methylation level and PM_2.5 species.
  
  Black participants exhibited stronger associations compared to White participants.
  
  Gene targets had relevance to pathways to basal cancers, platelet activation, signaling, and aggregation.

ABSTRACT

ABSTRACT

Minorities experience a disproportionate impact from PM_2.5 pollution, and biological mechanisms remain unclear. No studies have examined the epigenome-wide association between DNA methylation level and PM_2.5 species using longitudinal measurements of methylation among Blacks. This study aims to evaluate the relationship between exposure to PM_2.5 species and DNA methylation as well as assess possible race-specific effects. Genome-wide DNA methylation levels were profiled on 1,081 longitudinally followed participants (432 Black and 649 White adults) using the Infinium MethylationEPIC array. We examined the association between one-year average PM_2.5 species (black carbon, ammonium, nitrate, organic matter, sulfate, soil, and sea salt) exposure prior to exam year (Y)15 (2000-2001) and methylation at 841,639 CpG sites at Y15 and Y20 (2005-2006), respectively. In the association analysis of PM_2.5 species at Y15 with DNA methylation at Y20, methylation in 17 and 2 CpG sites, respectively, was significantly associated with nitrate and ammonium exposure. The magnitudes of coefficients of these significant CpG sites at Y20 increased by 2- to 48-fold compared to the cross-sectional association analysis of PM_2.5 species exposure prior to Y15 and DNA methylation at Y15. Black participants had stronger associations than White participants. Gene enrichment analysis indicated gene targets might be relevant to pathways including basal cancers, platelet activation, signaling, and aggregation. This study represents the first one to investigate the association between PM_2.5 species exposure and DNA methylation among Black and White adults using longitudinal measurement, highlighting the significance of considering race-specific epigenetic regulation in relation to air pollution-related health issues.

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Yao J., Zheng Y., Shen J., et al., (2024). Racial disparity in PM2.5 species and DNA methylation in CARDIA: An epigenome-wide association study. The Innovation Medicine 2(2): 100061. https://doi.org/10.59717/j.xinn-med.2024.100061

Yao J., Zheng Y., Shen J., et al., (2024). Racial disparity in PM_2.5 species and DNA methylation in CARDIA: An epigenome-wide association study. The Innovation Medicine 2(2): 100061. https://doi.org/10.59717/j.xinn-med.2024.100061

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Racial disparity in PM_2.5 species and DNA methylation in CARDIA: An epigenome-wide association study

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Racial disparity in PM2.5 species and DNA methylation in CARDIA: An epigenome-wide association study

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Racial disparity in PM_2.5 species and DNA methylation in CARDIA: An epigenome-wide association study