Illuminating a bacterial adaptation mechanism: Infrared-driven cell division in deep-sea hydrothermal vent environments
-
GRAPHICAL ABSTRACT
DownLoad:
Full size image
-
PUBLIC SUMMARY
-
The infrared (IR) radiation from hydrothermal vents impacted the composition of the microbial population.
A deep-sea bacterial strain was isolated from cultures enriched through IR irradiation.
High hydrostatic pressure (HHP) hindered bacterial peptidoglycan (PG) synthesis and cell division.
IR irradiation triggered PG synthesis and cell division under HHP, promoting bacterial growth.
-
-
ABSTRACT
Based on Planck's black-body radiation law, deep-sea hydrothermal vent chimneys emit light, predominantly infrared light, which potentially supports bacterial photosynthesis in this ecosystem independently of the solar energy. To investigate the impact of this geothermal light on bacterial growth, we collected samples from the Southwest Indian Ridge and demonstrated that infrared light alone promotes bacterial growth and alters population composition. The mechanism of infrared stimulated growth was analyzed by monitoring cell wall synthesis using the Tepidibacter hydrothermalis strain SWIR-1, which was isolated from cultures enriched through infrared irradiation. The results showed that the elevated hydrostatic pressure inhibited septal peptidoglycan synthesis and cell division, but had less effect on cell elongation, chromosome replication and segregation. The dominant cell shape was filaments with some swelling and inertness in cell wall synthesis depending on the level of pressure applied. Interestingly, irradiation with 880 nm infrared light effectively initiated septal synthesis and alleviated the obstruction. This revelation uncovers a novel adaptation mechanism involving infrared light for bacteria dwelling in deep-sea environments, and sheds light on the potential of infrared-mediated photobiomodulation. -
-
REFERENCES
[1] Du, M., Peng, X., Zhang, H., et al. (2021). Geology, environment, and life in the deepest part of the world's oceans. Innovation (Camb) 2(2): 100109. DOI: 10.1016/j.xinn.2021.100109. [2] Bartlett, D.H. (2002). Pressure effects on in vivo microbial processes. Biochim Biophys Acta 1595(1-2): 367−381. DOI: 10.1016/s0167-4838(01)00357-0. [3] Oger, P.M., and Jebbar, M. (2010). The many ways of coping with pressure. Research in Microbiology 161(10): 799−799. DOI: 10.1016/j.resmic.2010.09.017. [4] Black, S.L., Dawson, A., Ward, F.B., and Allen, R.J. (2013). Genes Required for Growth at High Hydrostatic Pressure in Escherichia coli K-12 Identified by Genome-Wide Screening. PloS one 8(9): e73995. DOI: 10.1371/journal.pone.0073995. [5] Li, A.Q., Zhang, W.J., Li, X.G., et al. (2023). Piezophilic Phenotype Is Growth Condition Dependent and Correlated with the Regulation of Two Sets of ATPase in Deep-Sea Piezophilic Bacterium Photobacterium profundum SS9. Microorganisms 11 (3). DOI: 10.3390/microorganisms11030637. [6] Yin, Q.J., Zhang, W.J., Qi, X.Q., et al. (2018). High hydrostatic pressure inducible trimethylamine N-oxide reductase iImproves the pressure tolerance of piezosensitive bacteria Vibrio fluvialis. Front Microbiol 8: 2646. DOI: 10.3389/fmicb.2017.02646. [7] Zobell, C.E., and Oppenheimer, C.H. (1950). Some effects of hydrostatic pressure on the multiplication and morphology of marine bacteria. J Bacteriol 60(6): 771−781. DOI: 10.1128/jb.60.6.771-781.1950. [8] Zobell, C.E., and Cobet, A.B. (1964). Filament formation by Escherichia coli at increased hydrostatic pressure. J Bacteriol 87(3): 710−719. DOI: 10.1128/jb.87.3.710-719.1964. [9] Ishii, A., Sato, T., Wachi, M., et al. (2004). Effects of high hydrostatic pressure on bacterial cytoskeleton FtsZ polymers in vivo and in vitro. Microbiology 150 (Pt 6):1965-1972. DOI: 10.1099/mic.0.26962-0. [10] Dick, G.J. (2019). The microbiomes of deep-sea hydrothermal vents: distributed globally, shaped locally. Nat Rev Microbiol 17(5): 271−283. DOI: 10.1038/s41579-019-0160-2. [11] He, X., Xu, T., Chen, C., et al. (2023). Same (sea) bed different dreams: Biological community structure of the Haima seep reveals distinct biogeographic affinities. The Innovation Geoscience 1(2): 100019. DOI: 10.59717/j.xinn-geo.2023.100019. [12] Van Dover, C.L., Reynolds, G.T., Chave, A.D., and Tyson, J.A. (1996). Light at deep-sea hydrothermal vents. Geophys. Res. Lett. 23(16): 2049−2015. DOI: 10.1029/96GL02151. [13] Nisbet, E.G., Cann, J.R., and Van Dover, C.L. (1995). Origins of photosynthesis. Nature 373: 479−480. DOI: 10.1038/373479a0. [14] Martin, W.F., Bryant, D.A., and Beatty, J.T. (2018). A physiological perspective on the origin and evolution of photosynthesis. FEMS Microbiol Rev 42(2): 205−231. DOI: 10.1093/femsre/fux056. [15] Xiao, W., He, H., Li, Y., and Konhauser, K. (2023). Abiotic oxidants promoted cyanobacteria’s evolution and Earth’s oxidation. The Innovation Geoscience 1: 100003. DOI: 10.59717/j.xinn-geo.2023.100003. [16] Yurkov, V.V., Krieger, S., Stackebrandt, E., and Beatty, J.T. (1999). Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181(15): 4517−4525. DOI: 10.1128/JB.181.15.4517-4525.1999. [17] Beatty, J.T., Overmann, J., Lince, M.T., et al. (2005). An obligately photosynthetic bacterial anaerobe from a deep-sea hydrothermal vent. Proceedings of the National Academy of Sciences of the United States of America 102(26): 9306−9310. DOI: 10.1073/pnas.0503674102. [18] Chen, H., Li, D.H., Jiang, A.J., et al. (2022). Metagenomic analysis reveals wide distribution of phototrophic bacteria in hydrothermal vents on the ultraslow-spreading Southwest Indian Ridge. Marine Life Science & Technology 4(2): 255−267. DOI: 10.1007/s42995-021-00121-y. [19] Dai, J., Li, X.G., Zhang, W.J., and Wu, L.F. (2023). Tepidibacter hydrothermalis sp. nov., a novel anaerobic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 73 (11). DOI: 10.1099/ijsem.0.006151. [20] Cutler, K.J., Stringer, C., Lo, T.W., et al. (2022). Omnipose: a high-precision morphology-independent solution for bacterial cell segmentation. Nature Methods 19(11): 1438−1448. DOI: 10.1038/s41592-022-01639-4. [21] Koch, E.W., and Rosolowsky, E.W. (2015). Filament Identification through Mathematical Morphology. arXiv 1507: 02289. DOI: 10.48550/arXiv.1507.02289. [22] Du, P., Kibbe, W.A., and Lin, S.M. (2006). Improved peak detection in mass spectrum by incorporating continuous wavelet transform-based pattern matching. Bioinformatics 22(17): 2059−2065. DOI: 10.1093/bioinformatics/btl355. [23] Botas, J., Rodríguez del Río, Á., Giner-Lamia, J., and Huerta-Cepas, J. (2022). GeCoViz: genomic context visualisation of prokaryotic genes from a functional and evolutionary perspective. Nucleic Acids Research 50(W1): W352−W357. DOI: 10.1093/nar/gkac367. [24] Li, X.-G., Dai, J., Zhang, W.-J., et al. (2023). Genome analysis of Tepidibacter sp. SWIR-1, an anaerobic endospore-forming bacterium isolated from a deep-sea hydrothermal vent. Marine Genomics 71: 101049. DOI: 10.1016/j.margen.2023.101049. [25] Egan, A.J.F., Errington, J., and Vollmer, W. (2020). Regulation of peptidoglycan synthesis and remodelling. Nat Rev Microbiol 18(8): 446−460. DOI: 10.1038/s41579-020-0366-3. [26] Kuru, E., Tekkam, S., Hall, E., et al. (2015). Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat Protoc 10(1): 33−52. DOI: 10.1038/nprot.2014.197. [27] Hsu, Y.P., Rittichier, J., Kuru, E., et al. (2017). Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem Sci 8(9): 6313−6321. DOI: 10.1039/c7sc01800b. [28] Megrian, D., Taib, N., Jaffe, A.L., et al. (2022). Ancient origin and constrained evolution of the division and cell wall gene cluster in Bacteria. Nature Microbiology 7(12): 2114−2127. DOI: 10.1038/s41564-022-01257-y. [29] Michalik, S., Reder, A., Richts, B., et al. (2021). The Bacillus subtilis Minimal Genome Compendium. ACS Synthetic Biology 10(10): 2767−2771. DOI: 10.1021/acssynbio.1c00339. [30] Tsai, S.R., and Hamblin, M.R. (2017). Biological effects and medical applications of infrared radiation. J Photochem Photobiol B 170: 197−207. DOI: 10.1016/j.jphotobiol.2017.04.014. [31] Sharma, S.K., Sardana, S., and Hamblin, M.R. (2023). Role of opsins and light or heat activated transient receptor potential ion channels in the mechanisms of photobiomodulation and infrared therapy. Journal of Photochemistry and Photobiology 13: 100160. DOI: 10.1016/j.jpap.2023.100160. [32] Dai, H., Shen, Q., Shao, J., et al. (2021). Small Molecular NIR-II Fluorophores for Cancer Phototheranostics. Innovation (Camb) 2(1): 100082. DOI: 10.1016/j.xinn.2021.100082. [33] Zhou, Y., Li, J., Tan, Q., et al. (2023). Soft electronics go for three-dimensional health monitoring in deep tissue. The Innovation Materials 1(2): 100022. DOI: 10.59717/j.xinn-mater.2023.100022. [34] Hamblin, M.R., Huang, Y.Y., and Heiskanen, V. (2019). Non-mammalian Hosts and Photobiomodulation: Do All Life-forms Respond to Light. Photochem Photobiol 95(1): 126−139. DOI: 10.1111/php.12951. -
ABOUT THIS ARTICLE
Cite this article:
Dai J., Li X.G., Zhang T.-Y., et al., (2024). Illuminating a bacterial adaptation mechanism: Infrared-driven cell division in deep-sea hydrothermal vent environments. The Innovation Geoscience 2(1): 100050. https://doi.org/10.59717/j.xinn-geo.2024.100050
Welcome!
To request copyright permission to republish or share portions of our works, please visit Copyright Clearance Center's (CCC) Marketplace website at marketplace.copyright.com.
Figures(7)
Article Metrics
Relative Articles
Cited by
Export File
Citation
Dai J., Li X.G., Zhang T.-Y., et al., (2024). Illuminating a bacterial adaptation mechanism: Infrared-driven cell division in deep-sea hydrothermal vent environments. The Innovation Geoscience 2(1): 100050. https://doi.org/10.59717/j.xinn-geo.2024.100050
Format
Content
-
Figure 1.
IR stimulated the growth of hydrothermal vent microorganisms
-
Figure 6.
Patterns of PG synthesis in SWIR-1 cells under HHP at 30 MPa
-
Figure 2.
HHP affected SWIR-1 PG synthesis and cell division
-
Figure 3.
Effect of HHP on FDAA incorporation and cell division
-
Figure 4.
IR triggers PG synthesis at septa
-
Figure 5.
Presentation of “SSCA” procedure used for high-throughput determination of cell size, fluorescence density and location
-
Figure 7.
DCW cluster gene composition
