Single-cell analysis reveals conserved regulons shaping muscle stem cell behavior during development and aging in mammals

Zishuai Wang; Wei Wang; Wangchang Li; Yilong Yao; Weiwei Liu; Zhonglin Tang

doi:10.59717/j.xinn-life.2024.100075

The Innovation Life > 2024 Vol. 2 > No. 2 > 100075

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Single-cell analysis reveals conserved regulons shaping muscle stem cell behavior during development and aging in mammals

1.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China
2.
Kunpeng Institute of Modern Agriculture at Foshan, Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Foshan 528226, China
3.
GuangXi Engineering Centre for Resource Development of Bama Xiang Pig, Bama 547500, China

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4These authors contributed equally to this work
Corresponding author: tangzhonglin@caas.cn
Received Date: April 22, 2024

Accepted Date: June 07, 2024

Published Online: June 08, 2024

The Innovation Life 2(2), https://doi.org/10.59717/j.xinn-life.2024.100075 | Cite this article

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1. A conserved developmental coordinate between human and mouse MuSCs sheds light on their shared biology.
  
  Conserved regulons enhance the understanding of MuSC behavior during development and aging.
  
  USF2 is a pivotal conserved regulon essential for muscle cell myogenesis and aging.

ABSTRACT

ABSTRACT

Muscle stem cells (MuSCs) play a pivotal role in skeletal muscle development, regeneration, and maintenance. Previous studies have extensively investigated the transcriptional changes in MuSCs during various developmental stages in mammals using single-cell RNA sequencing. However, a gap remains in cross-species integrative research examining the factors influencing MuSC activity during development and aging. To address this gap, we constructed a conserved single-cell landscape of MuSCs in mammals, encompassing their developmental and aging stages. Our findings unveil a conserved developmental coordinate between human and mouse MuSCs, shedding light on their shared biology. The single-cell coordinated gene association in pattern sets (scCoGAPS) algorithm and dynamic time warping algorithm were used to investigate the temporal dynamics of skeletal muscle regeneration related gene patterns. Additionally, we scrutinized the conservation of regulons, delineating groups of genes under the influence of common transcription factors, particularly emphasizing the identification of pivotal factors governing MuSC behavior during aging. Notably, we identified USF2, a conserved regulon, as a key regulator influencing muscle cell myogenesis and aging. This research provides critical insights into the conserved aspects that influence MuSC behavior and highlights the significance of USF2 in MuSC regulation. By unraveling the intricate mechanisms underlying MuSC development and aging, our study opens avenues for advancements in regenerative medicine and muscle-related therapeutics.

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Wang Z., Wang W., Li W., et al., (2024). Single-cell analysis reveals conserved regulons shaping muscle stem cell behavior during development and aging in mammals. The Innovation Life 2(2): 100075. https://doi.org/10.59717/j.xinn-life.2024.100075

Wang Z., Wang W., Li W., et al., (2024). Single-cell analysis reveals conserved regulons shaping muscle stem cell behavior during development and aging in mammals. The Innovation Life 2(2): 100075. https://doi.org/10.59717/j.xinn-life.2024.100075

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Single-cell analysis reveals conserved regulons shaping muscle stem cell behavior during development and aging in mammals

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